Bio-Trac Thursday Seminar Series – Special Topics in Biotechnology – April 30 – June 18, 12:15-12:45 – Open to the Scientific Community

Bio-Trac is pleased announce its “Thursday Seminar Series: Special Topics in Biotechnology” starting on April 30 from 12:15-12:45.  These twenty-five minute live webinars will feature experts, active research scientists who participate in the hands-on Bio-Trac training program, presenting on relevant topics related to their field of interest. These webinars are open to the scientific community but registration is required.

April 30, 2020
Dr. Serguei Kozlov, NIH/NCI
Useful Tips on Designing Successful CRISPR Experiments
This presentation will summarize the main molecular features and key applications of CRISPR/Cas9 genome editing technology. Representative examples of CRISPR experimental strategies will be discussed and some practical advices shared on how to design efficient CRISPR systems to achieve gene disruption or more subtle targeted alterations and insertions of desired DNA sequences.
May 7, 2020
Dr. Allissa Dillman, NIH/CIT
R – Harnessing the Power of the Tidyverse
This walk through presentation will demonstrate the ability to generate multiple plots with a few simple commands.
May 14, 2020
Dr. Fatah Kashanchi, GMU
Separating Viruses from Exosomes
Dr. Kashanchi will discuss the separation of virus away from exosomes using multiple models of virus infection including HIV-1.  Functional analysis of the exosomes will be discussed on recipient uninfected cells along with mechanisms of how these exosomes can control the immune cells by either cell death or reprogramming.  Finally, he will discuss the latest findings on how to separate Corona viruses away from exosomes since they have similar sizes coming out of infected cells.  This will aid in better understanding of the cytokine storm observed in patients post infection.
May 21, 2020
Dr. Elke Bergmann-Leitner, WRAIR
Rare Cell Detection by Flow Cytometry: Enables in-depth Characterization of Functional Lymphocyte Subsets
The webinar will describe the importance of sample size calculation for flow cytometric analysis and limitations of conventional flow cytometry regarding detection threshold and sensitivity. We will also discuss methods enabling rare cell detection and characterization of low frequency cell populations.
May 28, 2020
Dr. Richard Lee, JHU
Bisulfite Pyrosequencing: Quantitative Approach for Assessing DNA Methylation
There continues to be much interest in epigenetics as one of the primary mechanisms that underlie numerous physiological and pathological processes, as well how organisms interact with the environment. In this webinar, I will cover the basics of bisulfite pyrosequencing, which is a highly quantitative method for measuring DNA methylation. I will also provide a brief overview of DNA methylation and use examples from my work to demonstrate how pyrosequencing can be applied in biomedical research.
June 4, 2020
Dr. Kristen Bircsak, Mimetas
Modeling the prostate tumor microenvironment using organ-on-a-chip technology
Many of the current two-dimensional (2D) cell culture models used in prostate cancer research fail to recapitulate the prostate tumor microenvironment leading to low predictivity of the models. Physiologically based, 3D in vitro systems offer the potential to enhance the complexity and fidelity of 2D systems as they incorporate many components present in the in vivo tumor including extracellular matrix, stromal cell types, and perfusion. This presentation will review the setup and applications of a prostate tumor-on-a-chip model.
June 11, 2020
Dr. Michael Surace
Immuno-Oncology Multiplexing – Hyperplexing
June 18, 2020
Dr. Maria Hernandez, NIH/NCI/FNL
Sample Processing Considerations for Single Cell Sequencing – A Crucial Component of Experimental Design and Data Interpretation
A successful single cell study starts with a thoughtful consideration of how to prepare a cell or nuclei suspension. Choices on how to prepare sample may be intricately related to tissue type, study design and the experimental questions sought after. Knowledge of how samples were processed, including any enrichment or selection can influence data interpretation in important ways.
We look forward to when we are able to conduct our hands-on workshops again and continue to serve the scientific community. In the meanwhile, please join us for lunch and stimulating conversation.  Please consult the Bio-Trac 2020 workshop calendar for workshop updates.

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