Post-doctoral Research Fellow in Analytical and Bioprocess Research and Development – the successful candidate will be responsible for developing, optimizing and applying a variety of analytical methods designed to rapidly characterize N-linked oligosaccharide structures on test monoclonal antibodies and determine nucleotide sugar levels in cell culture samples. The candidate will also be responsible for employing cell and molecular biology techniques aimed at modulating N-linked oligosaccharide structures. The position is shared between the Analytical Research and Development and the Bioprocess Research and Development groups in the Biotherapeutics Pharmaceutical Sciences department. These groups are partnering in collaboration with an academic research lab in Cambridge, MA to understand the systems and synthetic biology concepts and tools required to control the N-glycan profile of monoclonal antibodies expressed in CHO production cells. The goal of the work is the development of methods in support of this collaboration. The candidate will be expected to evaluate existing methodology and then further develop, optimize and or apply as necessary to satisfy the analytical needs of the collaboration. The desired outcome is the publication of results in peer-reviewed journals and presentations at scientific conferences.
The qualified candidate will be responsible for developing, optimizing and applying a variety of analytical methods designed to rapidly characterize N-linked oligosaccharide structures on test monoclonal antibodies and determine nucleotide sugar levels in cell culture samples. Thorough research of contemporary literature regarding high throughput analysis of N-linked glycans and nucleotide sugars and application/advancement of state of the art techniques documented within the literature is expected. The candidate will be expected to assist in applying learning from the analytical analysis by designing and executing experiments using cell culture models to modulate N-linked glycans. The candidate will also be expected to work with cell line development colleagues at Pfizer or in an academic lab to develop sample handling procedures designed to minimize sample handling artifacts and generate samples that are most representative of the cell’s metabolic state. The qualified candidate will be expected to collaborate with multiple groups within Pfizer and with an external academic lab. The candidate will be expected to generate data and contribute to manuscripts for publication in peer-reviewed journals and to presentations at scientific conferences.
PhD in Chemistry, Biochemistry or related fields required. A strong background in N-linked glycan analysis and small molecule separations is required. This includes experience with high performance liquid chromatography and mass spectrometry at a minimum. Experience with high throughput analytics is desirable. A strong understanding of the glycosylation pathways in mammalian cell systems is required. A working knowledge of cell and molecular biology and cell culture techniques is desirable. Strong written and verbal communication skills are required. A strong history of publications in peer-reviewed journals and presentations at national conferences is desired.